https://journals.umt.edu.pk/index.php/CPR <p>Currents in Pharmacutical Research (CPR) is an International open access peer-reviewed journal that aims to publish high-quality research in the field of pharmaceutical sciences. The journal welcomes contributions from researchers worldwide and encourages interdisciplinary approaches that integrate pharmaceutical science with other related fields. The journal aims to be a valuable resource for the pharmaceutical industry, regulatory bodies, and healthcare professionals seeking to enhance their understanding of drug development, evaluation, and use.</p> University of Management and Technology, Lahore, Pakistan en-US Currents in Pharmaceutical Research 3007-3235 https://journals.umt.edu.pk/index.php/CPR/article/view/6441 <p>Folklorically, <em>Viola canescens</em> (VC) is used for the treatment of respiratory disorders, such as cough and asthma. The current study aimed to evaluate VC for anti-asthmatic and anti-tussive activities and isolate the bioactive compound. The plant was collected, extracted, and fractionated, with the isolation of compounds via column chromatography. Solvent extracts of VC were evaluated for anti-asthmatic and anti-tussive activities in animal models. The purified compound obtained from ethyl acetate fraction (EF) via column chromatography was identified by Mass and ¹H-NMR spectroscopic techniques. The EF (at 200 mg/kg/day for 21 days) significantly increased latency to pre-convulsive dyspnea (PCD) as compared to control. Similarly, mucous viscosity of the sensitized group was reduced significantly by EF (at 200 mg/kg). In isolated guinea pig’s ileum preparation, the EF (at 25 μg/ml) exhibited maximum relaxant activity (48.83 ± 1.5% inhibition at 0.8 ml) against histamine-induced contractions (<em>p</em>&lt;0.01). The citric acid-induced cough bouts in guinea pigs were significantly reduced by EF (58.13 ± 3.1% at 200 mg/kg). The EF also significantly inhibited the secretion of phenol red dye (43.02 ± 3.1% at 200 mg/kg). A flavonoid (quercetin) was isolated from the EF that may be probably responsible for anti-asthmatic and anti-tussive activities of the plant.&nbsp;</p> Abdullah Irfan Ullah Waqar Ahmad Muhammad Ibrar Bashir Ahmad Copyright (c) 2025 Currents in Pharmaceutical Research 2024-11-15 2024-11-15 2 2 01 21 10.32350/cpr.22.01