Physicochemical Evaluation of Streptomycin Sulphate using Simple and Newly Developed Spectrophotometric Methods

  • Ubed-Ur-Rehman Mughal Department of Pharmaceutics, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
  • Madan Lal Maheshwari Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
  • Abdul Rehman Uzaid Mughal Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
  • Fozia Rustamani Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand; and Department of Pharmaceutics, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
  • Muhammad Akram Khatri Department of Pharmacology, Faculty of Pharmacy, University of Sindh, Jamshoro, Sindh, Pakistan
  • Sara Aftab Institute of Biochemistry, Faculty of Natural Sciences University of Sindh, Jamshoro, Sindh, Pakistan
  • Saeed Ahmed Lakho Institute of Biochemistry, Faculty of Natural Sciences University of Sindh, Jamshoro, Sindh, Pakistan https://orcid.org/0000-0002-4732-3857
DOI: https://doi.org/10.32350/cpr.31.04
Keywords: Beer’s law, spectrophotometry, Steptomycin sulphate (STP), vanillin

Abstract

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Streptomycin sulphate (STP), an antibiotic that belongs to the aminoglycoside class, works by blocking the protein synthesis of the bacteria. This research presents a simple, fast, and cost-effective method for the analysis of the physicochemical parameters of STP used by three brands. The current study was conducted in two stages. In the first stage, physical properties such as color, pH, and weight variations of brands containing STP were evaluated using simple aesthetic procedures. In the second stage, chemical analysis was carried out using derivatization approach, followed by analysis with spectrophotometer. The derivatization of STP was carried out using vanillin as derivatizing agent. Beer’s law was followed in the range of 10-50 µg/ml, while calibration curve was obtained in the same range with R2value of 0.9993, which shows the linearity of the method. The analysis was carried out using the recovery method. There was 99-101% recovery of the drug using the new method with relative standard deviation ranging between 0.19-0.22%. The results showed that the new method is accurate and precise.

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Published
2025-05-26
How to Cite
Mughal, U.-U.-R., Maheshwari, M. L., Mughal, A. R. U., Rustamani, F., Khatri, M. A., Aftab, S., & Lakho, S. A. (2025). Physicochemical Evaluation of Streptomycin Sulphate using Simple and Newly Developed Spectrophotometric Methods . Currents in Pharmaceutical Research, 3(1), 69-82. https://doi.org/10.32350/cpr.31.04
Issue
Vol 3 No 1: Spring 2025
Section
Articles

Copyright (c) 2025 Currents in Pharmaceutical Research

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