The aim of the current study was to assess the perceived stress, life satisfaction and self-esteem of women studying in co-education and uni-gender institutions. The sample comprised a total of 200 female students, 100 from uni-gender institutions and 100 from co-education institutions. Convenience and purposive sampling techniques were used to collect the data. The standardized Urdu versions of Perceived Stress Scale, Satisfaction with Life Scale and Self-esteem Scale were used to collect the responses of students. SPSS (19th version) was used to analyze the data. Findings showed that women studying in uni-gender institutions are more stressed as compared to female students of co-education institutions but their self-esteem and life satisfaction remain unaffected. The results indicated that there is a significant difference between perceived stress, life satisfaction and self-esteem among female students. Moreover, they also revealed that stress and self-esteem affect the life satisfaction of women studying in co-education and uni-gender institutions. Furthermore, the results also depicted significant relationships between perceived stress, life satisfaction and self-esteem. The study will be helpful in improving the educational systems so that female students may enjoy a better learning environment which will boost up their confidence and self-esteem.