Evaluation of the Antidiabetic Activity of Asphodelus Tenuifolius in Normal and Alloxan-induced Diabetic Rats
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Asphodelus tenuifolius is traditionally used for the treatment of diabetes mellitus (DM). The current study aims to investigate the antidiabetic activity of Asphodelus tenuifolius in normal and alloxan-induced diabetic rats. Ethanolic and aqueous extracts of whole Asphodelus tenuifolius plant were prepared by using the maceration process. DM was induced by a single intraperitoneal injection of alloxan monohydrate (140 mg/kg b.w) in rats. Glibenclamide was used as the reference drug. In an acute study, ethanolic extract of Asphodelus tenuifolius (ATEE) and aqueous extract of Asphodelus tenuifolius (ATAqE) were administered in 200 and 400 mg/kg doses to normal and alloxan-induced diabetic rats. Both extracts (ATEE and ATAqE) significantly lowered the blood glucose level of both normal and diabetic treated rats in a concentration dependent fashion. However, ATEE produced prominent results at the dose of 400 mg/kg. In a fourteen-day study, ATEE considerably decreased the blood glucose level of alloxanized rats. The results were similar to the reference drug, that is, glibenclamide. In the prolonged study, the effects of ATEE on liver enzymes and hematological parameters of diabetic rats were also studied. Hb level and platelet count was increased in ATEE-treated diabetic rats as compared to diabetic control. However, it did not affect other hematological parameters. ATEE significantly decreased the ALP level as compared to diabetic control. Although, the test extract did not significantly alter the SGOT and SGPT levels. Further, the phytochemical testing of ATEE revealed the presence of alkaloids, flavonoids, tannins, and terpenoids. It was concluded that Asphodeleus tenifolius possesses antidiabetic activity. More comprehensive studies are needed in the future to explicate the mechanism of action and to characterize the phyto-components of this plant.
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Sikarwar MS, Patil M. Antidiabetic activity of Pongamia pinnata leaf extracts in alloxan-induced diabetic rats. Int J Ayurveda Res. 2010;1(4):199–204. https://doi.org/10.4103/0974-7788.76780
Patel D, Prasad SK, Kumar R, Hemalatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed. 2012;2(4):320–330. https://doi.org/10.1016/S2221-1691(12)60032-X
Rashid M, Bashir S, Mushtaq MN, et al. Comparative hypoglycemic activity of different extracts of Teucrium stocksianum in diabetic rabbits. Bangladesh J Pharmacol. 2013;8(2):186–193. https://doi.org/10.3329/bjp.v8i2.14519
Majeed Y, Shaukat MB, Abbasi KY, Ahmad MA. Indigenous plants of Pakistan for the treatment of diabetes: a review. Agrobiol Rec. 2021;4:44–63. https://doi.org/10.47278/journal.abr/2020.028
Aslam N, Janbaz KH, Jabeen Q. Hypotensive and diuretic activities of aqueous-ethanol extract of Asphodelus tenuifolius. Bangla J Pharmacol. 2017;11(4):830–837. https://doi.org/10.3329/bjp.v11i4.27131
Quattrocchi U. CRC world dictionary of medicinal and poisonous plants: common names, scientific names, eponyms, synonyms, and etymology (5 Volume Set): CRC press; 2012.
Qureshi R, Bhatti GR, Memon RA. Ethnomedicinal uses of herbs from northern part of Nara desert, Pakistan. Pak J Bot. 2010;42(2):839–851.
Kalim MD, Bhattacharyya D, Banerjee A, Chattopadhyay S. Oxidative DNA damage preventive activity and antioxidant potential of plants used in Unani system of medicine. BMC Complement Altern Med. 2010;10(1):e77. https://doi.org/10.1186/1472-6882-10-77
Pari L, Satheesh MA. Antidiabetic activity of boerhaavia diffusa L.: effect on hepatic key enzymes in experimental diabetes. J Ethnopharmacol. 2004;91(1):109–113. https://doi.org/10.1016/j.jep.2003.12.013
Jarald EE, Joshi SB, Jain DC, Edwin S. Biochemical evaluation of the hypoglycemic effects of extract and fraction of Cassia fistula Linn. in alloxan-induced diabetic rats. Indian J Pharm Sci. 2013;75(4):e427. https://doi.org/10.4103%2F0250-474X.119823
Alamgeer, Khan AQ, Ahmad T, et al. Phytochemical analysis and cardiotonic activity of methanolic extract of ranunculus muricatus linn. In isolated rabbit heart. Acta Pol Pharm. 2016;73(4):949–954.
Alamgeer, Mushtaq MN, Rashid M, et al. Evaluation of hypoglycemic activity of Thymus serpyllum Linn in glucose treated mice. Int J Basic Med Sci Pharm. 2014;3(2):33–36.
Ahmed MF, Kazim SM, Ghori SS, et al. Antidiabetic activity of Vinca rosea extracts in alloxan-induced diabetic rats. Int J Endocrinol. 2010;2010:e841090. https://doi.org/10.1155/2010/841090
Jorns A, Munday R, Tiedge M, Lenzen S. Comparative toxicity of alloxan, N-alkylalloxans and ninhydrin to isolated pancreatic islets in vitro. J Endocrinol. 1997;155:283–294.
Alamgeer MR, Mushtaq M, Malik M, et al. Pharmacological evaluation of antidiabetic effect of ethyl acetate extract of Teucrium stocksianum Boiss in alloxan-induced diabetic rabbits. J Anim Plant Sci. 2013;23(2):436–439.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;50(6):537–546.
Robertson RP, Harmon J, Tran PO, Tanaka Y, Takahashi H. Glucose toxicity in β-cells: Type 2 diabetes, good radicals gone bad, and the glutathione connection. Diabetes. 2003;52(3):581–587. https://doi.org/10.2337/diabetes.52.3.581
Peth JA, Kinnick TR, Youngblood EB, Tritschler HJ, Henriksen EJ. Effects of a unique conjugate of α-lipoic acid and γ-linolenic acid on insulin action in obese Zucker rats. Am J Physiol-Regul Integr Comp Physiol. 2000;278(2):R453–R459. https://doi.org/10.1152/ajpregu.2000.278.2.R453
Antia BS, Okokon JE, Umoh EE, Udobang JA. Antidiabetic activity of ethanolic leaf extract of Panicum maximum. Int J Drug Dev Res. 2010;2(3):488–492.
Rao BK, Kesavulu M, Giri R, Rao CA. Antidiabetic and hypolipidemic effects of Momordica cymbalaria Hook. fruit powder in alloxan-diabetic rats. J Ethnopharmacol. 1999;67(1):103–109. https://doi.org/10.1016/S0378-8741(99)00004-5
Eddine LS, Segni L, Ridha OM. In vitro assays of the antibacterial and antioxidant properties of extracts from Asphodelus tenuifolius Cav. and its main constituents: a comparative study. Int J Pharm Clin Res. 2015;7(2):119–125.
Safder M, Riaz N, Imran M, Nawaz H, Malik A, Jabbar A. Phytochemical studies on Asphodelus tenuifolius. J Chem Soci Pak. 2009;31(1):122–125.
Patel D, Shukla S, Gupta S. Apigenin and cancer chemoprevention: progress, potential and promise (review). Int J Oncol. 2007;30(1):233–245. https://doi.org/10.3892/ijo.30.1.233
Zhao B, Hall CA. Composition and antioxidant activity of raisin extracts obtained from various solvents. Food Chem. 2008;108(2):511–518. https://doi.org/10.1016/j.foodchem.2007.11.003
Ahmed N, Mahmood A, Tahir S, et al. Ethnomedicinal knowledge and relative importance of indigenous medicinal plants of Cholistan desert, Punjab Province, Pakistan. J Ethnopharmacol. 2014;155(2):1263–1275. https://doi.org/10.1016/j.jep.2014.07.007
Younis W, Schini-Kerth V, Junior AG, Nocchi SR, Silva DB, Roberts RE. Endothelium-independent vasorelaxant effect of Asphodelus tenuifolius Cav. via inhibition of myosin light chain kinase activity in the porcine coronary artery. J Ethnopharmacol. 2021;269:e113693. https://doi.org/10.1016/j.jep.2020.113693
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