Comparative Analysis of Methicillin-resistant Staphylococcus aureus through Whole Genome Sequencing (WGS) in the Largest Tertiary Care Hospital in Peshawar, Pakistan

  • Aman Ullah Institute of Paramedical Sciences, Khyber Medical University, Peshawar, Pakistan.
  • Muhammad Arif Khan Department of Medical Laboratory Technology, University of Haripur, Pakistan.
  • Saira Department of Zoology, University of Swabi, Pakistan
  • Ikram Ullah Department of Medical Lab. Technology, University of Haripur
  • Tanveer Tara Department of Medical Laboratory Technology, National Skills University, Islamabad, Pakistan.
  • Daud Ahmad Department of Medical Laboratory Technology, University of Haripur, Pakistan.
  • Muhammad Asif Zeb Institute of Paramedical Sciences, Khyber Medical University, Peshawar, Pakistan
DOI: https://doi.org/10.32350/cto.22.04
Keywords: methicillin-resistant, S. aureus, molecular typing, resistance genes, virulence genes, Whole Genome Sequencing (WGS)

Abstract

Abstract Views: 103

Methicillin-resistant Staphylococcus aureus (MRSA) is a globally disseminated antibiotic-resistant pathogen that causes mild to severe infections. The population structure of MRSA is highly clonal. Individual genetic clones harbor specific plasmids and determinants for antibiotic resistance and virulence. Therefore, whole genome sequencing (WGS) of five MRSA isolates of the predominant genetic lineage was performed to gain insight into the most prevalent endemic strains. Sequencing libraries were arranged in accordance with the Nextera XT DNA Library Prep Guide (Illumina). WGS was performed on Illumina NextSeq 500 platform with 2×151bp using a NextSeq Mid-Output Kit. The sequenced genome was characterized for spa type, MLST type, SCCmec type, plasmid, antibiotic resistance gene, and virulence gene. This characterization was performed by publicly available bioinformatics tools, available on the website of the Center for Genomic Epidemiology. All the isolates were characterized as ST8/t064-SCCmecIVa (2B) clones of MRSA. A total number of 13 resistance genes, 11 virulence genes, and 6 types of plasmids were identified in the sequenced isolates of MRSA. Furthermore, the sequenced isolates showed a similar pattern of distribution for antibiotic resistance genes and virulence genes. WGS revealed that the isolates were genetically closely related and showed a similar pattern of distribution for different genetic markers. Therefore, these strains could be an accidental case cluster or cluster of an outbreak.       

Downloads

Download data is not yet available.
Published
2022-12-28
How to Cite
Ullah, A., Khan, M. A., Saira, Ullah, I., Tara, T., Ahmad, D., & Zeb, M. A. (2022). Comparative Analysis of Methicillin-resistant Staphylococcus aureus through Whole Genome Sequencing (WGS) in the Largest Tertiary Care Hospital in Peshawar, Pakistan. Current Trends in OMICS, 2(2), 56-66. https://doi.org/10.32350/cto.22.04
Issue
Vol 2 No 2: Fall 2022
Section
Articles

Copyright (c) 2022 Aman Ullah, Muhammad Arif Khan, Saira, Ikram Ullah, Tanveer Tara, Daud Ahmad, Muhammad Asif Zeb

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Author(s) retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution (CC-BY) 4.0 License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.