Morphological and Genetic Identification of Head Taunsa Barrage Fish Species

  • Dr. Saima Naz Department of Zoology, Government Sadiq College Women University, Bahawalpur, Pakistan
  • Ahmad Manan Mustafa Chatha Department of Entomology, The Islamia University of Bahawalpur, Pakistan
  • Urooj Nazir Department of Zoology, The Islamia University of Bahawalpur, Pakistan
  • Syeda Saira Iqbal Department of Sustainable Development and Study Centre, Government College University, Lahore, Pakistan
  • Durali DANABAS Munzur University, Fisheries Faculty, TR62000, Tunceli, TURKEY
Keywords: biodiversity, COI barcode sequences, genetic identification, mitochondrial genome, Taunsa Barrage, taxonomic classification

Abstract

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The current study aims to construct a thorough barcoding reference database of fishes in the Head Taunsa Barrage and assess the usefulness of employing the COI gene for fish species identification. A total of 15 genera, 10 families, and 7 orders of fish were used to collect a total of 19 mitochondrial COI barcode sequences. These sequences had an average length of 800 base pairs. Within species, genera, families, orders, and classes, the average Kimura two-parameter (K2P) distances were 0.97%, 0.99%, 1.23%, and 1.26%, respectively. According to their taxonomic classification, species were commonly clustered in the K2P neighbor-joining trees based on the sequence. DNA barcoding was employed in this study to identify species with a high degree of accuracy. Moreover, it was concluded that COI sequencing can be used to recognize fish species.

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Published
2023-06-15
How to Cite
Naz, D. S., Chatha, A. M. M., Nazir, U., Iqbal, S. S., & DANABAS , D. (2023). Morphological and Genetic Identification of Head Taunsa Barrage Fish Species. Current Trends in OMICS, 3(1), 39-59. https://doi.org/10.32350/cto.31.03
Section
Articles