Phenotypic and Genotypic Detection of Virulence Factors Affecting Proteus mirabilis Clinical Isolates

  • Fawad Hayat Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan
  • Muddasir Khan Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan
  • Muhammad Umair Department of Medical Lab Technology, University of Haripur, Pakistan; Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan
  • Sajeela Akbar Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan
  • Rida Javed Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan
  • Syed Hussain Shah Department of Health and Biological Sciences, Abasyn University Peshawar, Pakistan
DOI: https://doi.org/10.32350/cto.31.05
Keywords: genotypic, phenotypic, polymerase chain reaction (PCR), Proteus mirabilis, virulence factors

Abstract

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The current study attempts the phenotypic and molecular detection of the virulence factors of Proteus mirabilis clinical isolates. A total of 600 urine samples were collected from urinary tract-infected patients at Khyber Teaching Hospital, Peshawar, Pakistan. P. mirabilis isolates were identified through different biochemical tests. Virulence factors including urease production, biofilm formation, and swarming phenomenon were determined by using different markers (ureC1, rsbA, and luxS genes), identified via the polymerase chain reaction (PCR) technique. Out of the selected samples, 95 samples (15.83%) were identified as P. mirabilis. The molecular study showed that all isolates (100%) possessed the ureC gene. Whereas, 90.52% and 92.63% of the isolates gave positive results for biofilm formation (rsbA gene) and swarming phenomenon (luxS gene), respectively. The phenotypic and molecular study of P. mirabilis virulence factors provides a better understanding of how P. mirabilis infection spreads. The results could be used for prevention and improvements in its clinical treatment.

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Published
2023-06-15
How to Cite
Hayat, F., Khan, M., Umair, M., Akbar, S., Javed, R., & Shah, S. H. (2023). Phenotypic and Genotypic Detection of Virulence Factors Affecting Proteus mirabilis Clinical Isolates. Current Trends in OMICS, 3(1), 73-85. https://doi.org/10.32350/cto.31.05
Issue
Vol 3 No 1 (2023): Spring
Section
Articles

Copyright (c) 2023 Fawad Hayat, MUDDASIR KHAN, Sajeela Akbar, Rida Javed, Syed Hussain Shah

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