Natural Synthesis of Silver Nanoparticles from Grape Fruit Juice and Estimation of Antimicrobial Activity

Mehreen Fatima; Azra  Quraishi; Maida  Akram

doi:10.32350/BSR.0302.i

Mehreen Fatima University of Management and Technology, Lahore and University of the Punjab, Lahore, Pakistan https://orcid.org/0000-0001-8019-229X
Azra Quraishi University of Management and Technology, Lahore and University of the Punjab, Lahore, Pakistan https://orcid.org/0000-0002-7293-2961
Maida Akram Faculty of Pharmacy, Punjab University College of Pharmacy, Lahore, Pakistan

DOI: https://doi.org/10.32350/BSR.0302.i

Keywords: antimicrobial effect, green chemistry, grape fruit, nanomedicine, silver nanoparticles

Abstract

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Silver has been known for its antimicrobial activity for a very long time. The formulation of silver particles that range from 1-100nm in size makes it even more potent in terms ofinducingantimicrobial effects.Green chemistry has started toinfluencethe field of biochemical research. Silver Nanoparticles(Ag-NPs)synthesized through the green synthesis method provide a cheap and ecofriendlywayof nanoparticle preparation. Theaim of the current study is to prepare the green synthesis of Ag-NPsusing tomato juice as a reducing and capping agent and the evaluation of its antimicrobial activity. The stability and conformation ofAg-NPs was determined usingUV-visible spectroscopy. The antimicrobial activity of synthesizedAg-NPs was determined against E.coli DH5α.Ultraviolet spectroscopic analysis offered peak at 400 nm whichindicatedthe production of Ag-NPs of adequate size. E.coli DH5αdecreasedconsiderably upon the introduction of Ag-NPsto the bacterial inoculum. On increasing the concentration of Ag-NPs,an increase in the zone of inhibition was recorded. For 70μg/ml of Ag-NPs the zone of inhibition was 0.5 cm, while 0.6 cm, 0.7 cm and 0.7cm wererecorded for 100μg/ml, 150μg/ml and 200μg/ml of Ag-NPs,respectively. The efficacy of the antimicrobial activity of Ag-NPsderived from tomato juice proves its potential use in pharmaceutical and medicinal industries for the synthesis of nanomedicine.

Keywords: antimicrobial effect, grapefruit, green chemistry, nanomedicine, silver nanoparticles (Ag-NPs)

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