Identification and Characterization of Urinary Tract Infectious Bacteria and its Antibiotic Sensitivity

Madeeha Mehboob; Muhammad  Hakim; Obaid Ullah; Sumaira Salahuddin  Lodhi; Muhammad  Anees; Irum Khalil; Malik Nawaz Shuja

doi:10.32350/BSR.0303.04

Madeeha Mehboob Department of Microbiology, Kohat University of Science & Technology, Kohat-26000 Khyber Pakhtunkhwa, Pakistan
Muhammad Hakim Department of Microbiology, Kohat University of Science & Technology, Kohat-26000 Khyber Pakhtunkhwa, Pakistan
Obaid Ullah Department of Microbiology, Kohat University of Science & Technology, Kohat-26000 Khyber Pakhtunkhwa, Pakistan
Sumaira Salahuddin Lodhi Departemnt of Biochemistry, Hazara University, Mansehra, Pakistan
Muhammad Anees Department of Microbiology, Kohat University of Science & Technology, Kohat-26000 Khyber Pakhtunkhwa, Pakistan
Irum Khalil Department of Biotechnology, Abdul Wali Khan University of Maran (AWKUM), Mardan, Pakistan
Malik Nawaz Shuja Department of Microbiology, Kohat University of Science & Technology, Kohat-26000 Khyber Pakhtunkhwa, Pakistan https://orcid.org/0000-0002-8255-7675

DOI: https://doi.org/10.32350/BSR.0303.04

Keywords: antimicrobial sensitivity, E. coli, K. pneumoniae, Urinary tract infection, uropathogens

Abstract

Abstract Views: 385

Etiological patterns of uropathogens are different in different geographical regions due to the continuous evolution of bacteria, antibiotic sensitivity patterns and their misuse and overuse. Therefore, it is important to know the antibiotic susceptibility patterns for the prescription of suitable antibiotics. This study was conducted to determine the prevalence of uropathogens and their antimicrobial sensitivity patterns in the Kohat region of Pakistan. In this study, 100 samples were collected from both male and female subjects of all ages. Out of these 100 samples, 70 samples contained microbes. In 30 samples, no microbial growth was recorded. The percentages of positive culture from male and female subjects were 57% and 43%, respectively. Both Gram (+) and Gram (-) bacteria were found in UTI. Among them E.coli (34.21%) was predominant, followed by K. pneumoniae (10.52%), P. aeruginosa (9.21%), K. oxytoca (6.57%), C. albicans (5.26%), E. faecium (5.26%), E. faecalis (3.94%), S. aureus (3.94%), E. cloacea (2.63%), C. freundii (2.63%), P. mirabalis (2.63%) and A. baumannii (1.31%). Many of the isolates showed resistance to commonly used antibiotics. The sensitivity percentage of commonly used antibiotics against both Gram (+) and Gram (-) bacteria are as follows: Ampicillin (13%), Ceftriaxone (25%), Amikacin (77%), Gentamicin (41%), Augmentin (44.77%), Fosfomycin (64%), Cotrimoxazole (36%), Nitrofurantoin (68%), Ciprofloxacin (37%), Imipenem (78%), Meropenem (67%), Cefepime (25%) and Tetracycline (40%). The most effective antibiotics against both Gram (+) and Gram (-) bacteria were Fosfomycin, Imipenem, Meropenem Amikacin and Nitrofurantoin. In light of the findings of this study, it is strongly recommended to find new antimicrobial compounds. Moreover, it is imperative to evaluate the resistant patterns at genomic and proteomic levels to discover the genes responsible for antibiotic resistant patterns.

Keywords: antimicrobial sensitivity, E. coli, K. pneumonia, urinary tract infection, uropathogens

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