Monocyte-to-Lymphocyte Ratio (MLR) as a Possible Prognostic Marker of Latent Tuberculosis (LTBI) among Household Contacts of Active Tuberculosis (TB) PatientsTuberculosis (TB) is endemic in many developing countries including Pakistan. It is a leading cau
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Tuberculosis (TB) is endemic in many developing countries including Pakistan. It is a leading cause of death from a single infectious agent worldwide. Identification and early treatment of latent conditions help reduce the complications associated with TB. However, the identification of individuals with latent infection is a time taking and expensive process. According to previous studies, a promising and cheap biomarker of TB may be the monocyte-to-lymphocyte ratio (MLR). It may indicate a body’s immune response to Mycobacterium tuberculosis. Since household contacts of tuberculosis (TB) patients have an increased risk of latent tuberculosis (LTBI), using the established diagnostic procedures as well as checking their MLR might help determine if they contracted LTBI or not. We conducted across-sectional study to determine if MLR could be used to identify LTBI among household contacts of patients with active tuberculosis. Out of the 100 subjects selected for this study, about 40 patients were recently diagnosed with active tuberculosis, 40 were close contacts of these patients, while20 were chosen to be controls. The mean was 0.165, 0.06 (range 0.03–0.08), and 0.04 (0.02–0.04) inpatients with active tuberculosis, close contacts of patients, and control subjects, respectively. Hence, it was determined that MLR (> 0.6 %)is a significant predictor for LTBIand can be used to diagnose it in close contacts of TB patients. It was additionally observed that patients over the age of 50 with pulmonary tuberculosis have higher MLR.
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Zaidi SM, Waseem HF, Ansari FA, Irfan M, Fahim S, Ahmad M. Sample size estimation of diagnostic test
studies in health sciences. In14th Int Conference on 2016 (p. 239).
Al Hajoj S, Varghese B, Datijan A, et al. Interferon gamma release assay versus tuberculin skin testing among healthcare workers of highly diverse origin in a moderate tuberculosis burden country. PloSOne. 2016;11(5):0154803.https://doi.org/ 10.1371/journal.pone.0154803 DOI: https://doi.org/10.1371/journal.pone.0154803
Sadaf R, Munir T, Farrukh S, Abbasi S. Prevalence of latent tuberculosis infection in healthcare workers in tertiary care hospitals of Pakistan.Pak J Med Sci. 2020;36(2):198-202. https://doi.org/10.12669/pjms.36.2. 936 DOI: https://doi.org/10.12669/pjms.36.2.936
Wang J, Yin Y, Wang X, et al. Ratio of monocytes to lymphocytes in peripheral blood in patients diagnosed with active tuberculosis.Braz J Infect Dis 2015;19(2):125-31.https://doi.org/ 10.1016/j.bjid.2014.10.008 DOI: https://doi.org/10.1016/j.bjid.2014.10.008
Sibley L, Gooch K, Wareham A, et al. Differences in monocyte: lymphocyte ratio and Tuberculosis disease progression in genetically distinct populations of macaques. Sci Rep. 2019;9(1):1-9. DOI: https://doi.org/10.1038/s41598-019-39819-6
Yang J, Zhang L, Yu C, Yang XF, Wang H. Monocyte and macrophage differentiation: circulation inflammatory monocyte as biomarker for inflammatory diseases. Biomark Res. 2014;7(2):1-2.https://doi.org/10. 1186/2050-7771-2-1 DOI: https://doi.org/10.1186/2050-7771-2-1
Esmail H, Barry III CE, Wilkinson RJ. Understanding latent tuberculosis: The key to improved diagnostic and novel treatment strategies. Drug
BioScientific Review Volume 4 Issue 1, 2022 DOI: https://doi.org/10.37435/nbrvol4.issue1
Monocyte-to-Lymphocyte Ratio… Discov Today. 2012;17(9-10):51421.https://doi.org/10.1016/j.drudis.201 1.12.013
Ahmad S. Pathogenesis, Immunology, and Diagnosis of Latent Mycobacterium Tuberculosis Infection. Clin& Develop Immunol. 2010;2011:1–2. DOI: https://doi.org/10.1155/2011/814943
Mayito J, Meya DB, Rhein J, Sekaggya-Wiltshire C. Utility of the monocyte to lymphocyte ratio in diagnosing latent tuberculosis among HIV-infected individuals with a negative tuberculosis symptom screen. PLoS One. 2020;15(11):e0241786.https://doi.org/ 10.1371/journal.pone.0241786 DOI: https://doi.org/10.1371/journal.pone.0241786
Yang J, Lee S, Oh S, et al. The risk of active tuberculosis among individuals living in tuberculosis-affected households in the Republic of Korea, 2015. PLoS ONE 2019; 14(12):e0225744. https://doi.org/ 10.1371 DOI: https://doi.org/10.1371/journal.pone.0225744
Simon D, Simon HU, Yousefi S. Extracellular DNA traps in allergic, infectious, and autoimmune diseases. Allergy. 2013;68(4):409–16. DOI: https://doi.org/10.1111/all.12111
Marahatta SB, Yadav RK, Giri D, et al. Barriers in the access, diagnosis and treatment completion for tuberculosis patients in central and western Nepal: A qualitative study among patients, community members and health care workers. PLoS ONE 2020;15(1):e0227293.https://doi.org/1 0.1371/journal.pone.0227293 13. Watkins RE, Plant AJ. Does smoking explain sex differences in the global tuberculosis epidemic? Epidemiol Infect. 2006;134:333–9. DOI: https://doi.org/10.1371/journal.pone.0227293
Lee H, Kim J, Ae Kang Y, et al. In vitro Mycobacterial Growth Inhibition in South Korean Adults with Latent TB Infection.Front Immunol. 2019;10:896https://doi.org/10.3389/fi mmu.2019.00896 DOI: https://doi.org/10.3389/fimmu.2019.00896
La Manna MP, Orlando V, Dieli F, et al. Quantitative and qualitative profiles of circulating monocytes may help identifying tuberculosis infection and disease stages. PLoS One. 2017;12(2):e0171358. https://doi.org/ 10.1371/journal.pone.0171358 DOI: https://doi.org/10.1371/journal.pone.0171358
Philips JA, Ernst JD. Tuberculosis pathogenesis and immunity, Annual Review of Pathology: Mech Dis. 2012;7(1):353–384 DOI: https://doi.org/10.1146/annurev-pathol-011811-132458
Wang W, Wang L, Liu YY, et al. Value of the Ratio of Monocytes to Lymphocytes for Monitoring Tuberculosis Therapy. Canadian J Infectious Dis Med Microbiol 2019;2019:1-5. DOI: https://doi.org/10.1155/2019/3270393
Naranbhai V, Fletcher HA, Tanner R, et al. Distinct transcriptional and antimycobacterial profiles of peripheral blood monocytes dependent on the ratio of monocytes: lymphocytes. EBioMed. 2015;2(11):1619-26. https://doi.org/10.1016/j.ebiom.2015.0 9.027 DOI: https://doi.org/10.1016/j.ebiom.2015.09.027
Liu C, Liu H, Ge B. Innate immunity in tuberculosis: host defense vs pathogen evasion. Cell MolImmunol 2017;14:963–975. https://doi.org/10.1038/cmi.2017.88 DOI: https://doi.org/10.1038/cmi.2017.88
Rakotosamimanana N, Richard V, Raharimanga V, Gicquel B, Doherty M, Zumla A. Biomarkers for risk of 10 Department of Life Sciences Volume 4 Issue 1, 2022
Khurshid et al.
developing active tuberculosis in contacts of TB patients. Eur Respir J 2015;46(4):1095-103. DOI: https://doi.org/10.1183/13993003.00263-2015
Liana P, Brestilova B, Rahadiyanto KY. The ratio of monocytes to lymphocytes accuracy as tuberculosis predictor. InJournal of Physics: Conference Series 2019 Jul 1 (Vol. 1246, No. 1, p. 012024). IOP Publishing. DOI: https://doi.org/10.1088/1742-6596/1246/1/012024
De Martino M, lodi L, Galli L, Chiapinni E. Immune Response to Mycobacterium tuberculosis: A Narrative Review. FrontPediatr. 2019;7:1-8. https://doi.org/10.3389/ fped.2019.00350 DOI: https://doi.org/10.3389/fped.2019.00350
Reece ST, Vogelzang A, Tornack J, et al. Mycobacterium TuberculosisInfected Hematopoietic Stem and Progenitor Cells Unable to Express Inducible Nitric Oxide Synthase Propagate Tuberculosis in Mice. J Infect Dis. 2018;217(10):1667-1671. https://doi.org/10.1093/infdis/jiy041 DOI: https://doi.org/10.1093/infdis/jiy041
Iqbal S, Ahmad U, Zaidi SBH. Monocyte Lymphocyte Ratio as a Possible Prognostic Marker in Ant tuberculous Therapy. J Rawal Med College. 2014;18(2):178-181.
Tabassum MN, Muhammad AK, Afzal S, Gilani A, Gureja AW, Tabassum S. Demographic Characteristics of Tuberculosis Patients at Public Sector Health Facilities in Lahore, Pakistan. Ann of King Edward Med Univ. 2018;24:16.https://doi.org/10.21649/akemu.v24i 1.2309 DOI: https://doi.org/10.21649/akemu.v24i1.2309
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