The residents of remote areas in developing countries mostly rely on traditional plants to cure
different ailments. Berberis royleana is a rare species among the genus Berberis which
commonly comprises 500 species distributed in Nepal, Afghanistan, Siberia, and other parts of
the world. Berberis are used to make herbal medication to fight against infections, ocular
trachoma, AIDS, and diarrhea. The current study was carried out to explore the in vitro
insecticidal activity of B. royleana in various extracts by direct contact application process
against the insect Callosobruchus maculatus. The maximum mortality rate of 60% was recorded
by water fraction, whereas methanolic fraction had 50% mortality, n-Hexane had 40% mortality,
while ethyl acetate and chloroform both had 30% mortality at 72 hours. Thus, this study
indicated that from the medicinal point of view this specie is very significant, with a great
potential for insecticidal activities against C. maculatus. It is further suggested that B. royleana
could be used as a good insecticidal agent against dengue and malarial vectors. In addition, the
side effects like toxicity could be addressed to avoid any mishaps.
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