Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

  • Malik Shuja Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan
  • Hassan Riaz Department of Plant Pathology, Muhammad Nawaz Shareef University of Agriculture, Multan
  • Muhsin Jamal Department of Microbiology, Abdul Wali Khan Universit y, Mardan, Pakistan
  • Muhammad Imran Department of Microbiology, University of Health Sciences, Lahore, Pakistan
Keywords: aminoglycoside, cereals, genetic transformation, plasmid, tissue culture

Abstract

Abstract Views: 397

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

 

Copyright (c) 2021 Malik Nawaz Shuja, Hasan Riaz, Muhsin Jamal, Muhammad Imran

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Published
2021-05-07
How to Cite
Shuja, M., Riaz, H., Jamal, M., & Imran, M. (2021). Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?. BioScientific Review, 3(1), 01-05. https://doi.org/10.32350/BSR.0301.i
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