Phytoremediation of Lead Contaminated Soil Using Sorghum Plant in Association with Indigenous Microbes

  • Iqra Tabassum Department of Biology, Lahore Garrison University, Pakistan
  • Sumaira Mazhar Department of Biology, Lahore Garrison University, Pakistan
  • Beenish Sarfraz Department of Biology, Lahore Garrison University, Pakistan
Keywords: bioremediation, heavy metals, lead, phytoremediation, sorghum

Abstract

Abstract Views: 118

Heavy metals are discharged in large quantities in both land and water bodies, causing long-term damage to living organisms. Phytoremediation is an effective way to address this problem. The goal of this study was to identify how lead resistant microorganisms affect the growth of sorghum plant, both in the presence and absence of lead. For this purpose, lead resistant microbes were isolated to investigate the growth and concentration of lead in the sorghum plant. Isolated species were inoculated with lead containing media in different concentrations, such as 300, 400, 500, and 600 µg/ml concentrations. Highly lead resistant bacterial isolates were selected and inoculated with sorghum seeds under typical environmental conditions in small pots, with and without lead contamination (300 mg/Kg). In the presence of lead resistant bacteria, efficient growth was observed with less concentration of lead in the plants. Promising results were observed in the presence of GS3 and IS2 isolates. The current study showed that lead tolerant bacterial isolates are very helpful to degrade lead when grown with sorghum seeds. Furthermore, it also enhances the growth of sorghum plant.

Downloads

Download data is not yet available.

References

Shafiq M, Iqbal M Z, Mohammad A. Effect of lead and cadmium on germination and seedling growth of Leucaena leucocephala. J Appl Sci Environ Manag. 2008;12(3):121-127. https://doi.org/10.33628/jpd.2020.27.1.121

Heidari M, Sarani S. Effects of lead and cadmium on seed germination, seedling growth and antioxidant enzymes activities of mustard (Sinapis arvensis L.). ARPN J Agric Biolog Sci. 2011;6(1):44-47. https://doi.org/10.1007/s00264-011-1239-1

Mani D, Kumar C, Patel NK. Integrated micro-biochemical approach for phytoremediation of cadmium and lead contaminated soils using Gladiolus grandiflorus L cut flower. Ecotoxicol Environ Saf. 2016;124:435-446.

https://doi.org/10.1016/j.ecoenv.2015.11.016

Li Y, Ali A, Jeyasundar PGSA, et al. Bacillus subtilis and saponin shifted the availability of heavy metals, health indicators of smelter contaminated soil, and the physiological indicators of Symphytum

officinale. Chemosphere. 2021;e131454. https://doi.org/10.1016/j.chemosphere.2021.131454

Zhuang P, Wensheng SHU, Zhian LI, Bin LIAO, Jintian LI, Jingsong SHAO. Removal of metals by sorghum plants from contaminated land. J Environ Sci. 2009;21(10):1432-1437.

https://doi.org/10.1016/s1001-0742(08)62436-5

Tüzen M. Determination of heavy metals in soil, mushroom and plant samples by atomic absorption spectrometry. Microchem J. 2003;74(3):289-297. https://doi.org/10.1016/s0026-265x(03)00035-3

Rachit K, Verma KS, Meena T, Yashveer V, Shreya H. Phytoextraction and bioconcentration of heavy metals by Spinacia oleracea grown in paper mill effluent irrigated soil. Nature Environ Poll Technol. 2016;15:817- 824.

https://doi.org/10.1007/s12355-012-0194-5

Singh TB, Kumar S, Singh DK, Khirwar SS, Agnihotri RK. Toxicity of Cr and Pb during vegetative growth of Sesamum indicum L. J Ind Botan Soc. 2019;98(3and4):219-226. https://doi.org/10.5958/2455-

2019.00027.5

Syed-Ab-Rahman SF, Carvalhais LC, Chua E, Xiao Y, Wass TJ, Schenk PM. Identification of soil bacterial isolates suppressing different Phytophthora spp. and promoting plant growth. Front Plant Sci. 2018;9:e1502. https://doi.org/10.3389/fpls.2018.01502

Amin H, Arain BA, Jahangir TM, Abbasi MS, Amin F. Accumulation and distribution of lead (Pb) in plant tissues of guar (Cyamopsis tetragonoloba L.) and sesame (Sesamum indicum L.): profitable phytoremediation with

biofuel crops. Geol Ecol Landscap. 2018;2(1):51-60. https://doi.org/10.1080/24749508.2018.1452464

Sahib MR, Pervaiz ZH, Williams MA, Saleem M, DeBolt S. Rhizobacterial species richness improves sorghum growth and soil nutrient synergism in a nutrient-poor greenhouse soil. Sci Rep. 2020;10(1):1-13. https://doi.org/10.1038/s41598-020-72516-3

Shen, Z. G., Li, X. D., P, C. C., Chen, H. M., & Chua, H. Lead phytoextraction from contaminated soil with high‐biomass plant species. J Environ Qual. 2002;31(6):1893-1900. https://doi.org/10.2134/jeq2002.1893

Wang X, Chen C, Wang J. Cs phytoremediation by Sorghum bicolor cultivated in soil and in hydroponic system. Int J Phytoremed. 2017;19(4):402-412. https://doi.org/10.1080/15226514.2016.1244158

Prakash G, Soni R, Mishra R, Sharma S. Role of plant-microbe interaction in phytoremediation. In: Kumar M, Muthusamy A, Kumar V, Bhalla-Sarin N. eds. vitro plant breeding towards novel agronomic traits. Springer;2019. https://doi.org/10.1007/978-981-32-9824-8_6

Verma R, Kumar R, Nath A. Drought resistance mechanism and adaptation to water stress in sorghum. Int J Bio-Res Stress Manag. 2018;9(1):167-172. https://doi.org/10.23910/ijbsm/2018.9.1.3c0472

Published
2022-09-15
How to Cite
1.
Iqra Tabassum, Sumaira Mazhar, Beenish Sarfraz. Phytoremediation of Lead Contaminated Soil Using Sorghum Plant in Association with Indigenous Microbes. Sci Inquiry Rev. [Internet]. 2022Sep.15 [cited 2024Nov.21];6(3):79-3. Available from: https://journals.umt.edu.pk/index.php/SIR/article/view/1854
Section
Orignal Article