Green Synthesis of Mixed Metal Oxide (MnO, CuO, ZnO) Nanoparticles (NPs) using Rose Petal Extract: An investigation of their Antimicrobial and Antifungal Activities

  • Syeda Shaista Gillani Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
  • Sana Khan Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
  • Rabia Nazir Applied Chemistry Research Centre (ACRC), PCSIR Labs Complex, Lahore, Pakistan
  • Aisha Waheed Qurashi Department of Biology, Lahore Garrison University, Lahore, Pakistan
Keywords: antifungal, antimicrobial, green synthesis, mixed metal oxides nanoparticles (MONPs), rose petals extracts


Abstract Views: 461

In this study, mixed metal oxide (CuO, ZnO, and MnO) nanoparticles (NPs) were synthesized via the green process, which is considered to be simple, cost-effective, eco-friendly, and non-toxic. During the green synthesis method, rose petal extract was used as a reducing and capping agent, while salt solutions (CuCl2, MnCl2, and ZnSO4) were used for the bio-reduction of metal precursors, leading to the formation of mixed metal oxide (CuO, ZnO, MnO) NPs. UV-Visible and FTIR spectroscopy were used for the analysis of mixed metal oxide (CuO, ZnO, MnO) NPs. The results showed maximum absorbance of the mixed metal oxide in the range of 280 to 370 nm. The presence of particular peaks in FTIR verified the synthesis of mixed metal oxide nanoparticles. Subsequently, the antimicrobial and antifungal activities of mixed metal oxide (CuO, ZnO, MnO) NPs were tested by using the disc diffusion method and well diffusion method. Mixed metal oxide (CuO, ZnO, MnO) NPs displayed antimicrobial activity against Escherichia coli and antifungal activity against Candida albicans, Curvularia lunata, Aspergillus niger, and Trichophyton simii. Hence, these mixed metal oxide (CuO, ZnO, MnO) NPs can be effectively used in the pharmaceutical sector.

Keywords: antifungal, antimicrobial, green synthesis, mixed metal oxides nanoparticles (MONPs), rose petals extracts

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Hornyak GL, Dutta J, Tibbals HF and Rao A (2008). Introduction to nanoscience, CRC press.

Salam HA, Rajiv P, Kamaraj M, Jagadeeswaran P, Gunalan S and Sivaraj R (2012). Plants: green route for nanoparticle synthesis. Int Res J Biol Sci 1(5): 85-90.

Doble M, Rollins K and Kumar A (2010). Green chemistry and engineering, Academic Press.

Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK. Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J Nanotechnol. 2018;9:1050-1074. Published 2018 Apr 3. doi:10.3762/bjnano.9.98

Jain N, Bhargava A, Majumdar S, Tarafdar J and Panwar J (2011). Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: a mechanism perspective. Nanoscale 3(2): 635-641.

Ivanković A, Dronjić A, Bevanda AM and Talić S (2017). Review of 12 principles of green chemistry in practice. Int J Sustain Green Energy 6(3): 39-48.

Hirulkar N and Agrawal M (2010). Antimicrobial activity of rose petals extracts against some pathogenic bacteria. Int. J. Pharm. Biol. Arch 1(5): 478-484.

. Villanueva A, De La Presa P, Alonso J, Rueda T, Martinez A, Crespo P, Morales M, Gonzalez-Fernandez M, Valdes J and Rivero G (2010). Hyperthermia HeLa cell treatment with silica-coated manganese oxide nanoparticles. The Journal of Physical Chemistry C 114(5): 1976-1981.

Shohayeb M, Abdel-Hameed E-SS, Bazaid SA and Maghrabi I (2014). Antibacterial and antifungal activity of Rosa damascena MILL. essential oil, different extracts of rose petals. Global Journal of Pharmacology 8(1): 1-7.

Rangahau MK. (2001). Rose-Rosa damascena'Trigintipetala. Crop Food Res Broad Sheet 29.

Rusanov K, Kovacheva N, Atanassov A and Atanassov I (2009). Rosa damascena Mill., the oil-bearing Damask rose: genetic resources, diversity and perspectives for molecular breeding. Floriculture Ornamental Biotech 3: 14-20

Basim E and Basim H (2003). Antibacterial activity of Rosa damascena essential oil. Fitoterapia 74(4): 394-396.

Leenen R, Roodenburg A, Tijburg L and Wiseman S (2000). A single dose of tea with or without milk increases plasma antioxidant activity in humans. European Journal of Clinical Nutrition 54(1): 87-92.

Jafari M, Zarban A, Pham S and Wang T (2008). Rosa damascena decreased mortality in adult Drosophila. Journal of medicinal food 11(1): 9-13.

Crespo M, Galvez J, Cruz T, Ocete M and Zarzuelo A (1999). Anti-inflammatory activity of diosmin and hesperidin in rat colitis induced by TNBS. Planta medica 65(07): 651-653.

Ren W, Qiao Z, Wang H, Zhu L, and Zhang L (2003). Flavonoids: promising anticancer agents. Medicinal research reviews 23(4): 519-534.

Tabrizi H, Mortazavi S and Kamalinejad M (2003). An in vitro evaluation of various Rosa damascena flower extracts as a natural antisolar agent. International journal of cosmetic science 25(6): 259-265.

Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. P T. 2015;40(4):277-283.27. 2012.

Tanwar J, Das S, Fatima Z, Hameed S. Multidrug resistance: an emerging crisis. Interdisciplinary perspectives on infectious diseases. 2014 Oct;2014.

Tzialla C, Borghesi A, Perotti GF, Garofoli F, Manzoni P, Stronati M. Use and misuse of antibiotics in the neonatal intensive care unit. The Journal of Maternal-Fetal & Neonatal Medicine. 2012 Oct 1;25(sup4):27-9.

Dadgostar P. Antimicrobial Resistance: Implications and Costs. Infect Drug Resist. 2019;12:3903-3910. Published 2019 Dec 20. doi:10.2147/IDR.S234610

Cho JI, Lee SH, Lim JS, Koh YJ, Kwak HS and Hwang IG (2011). Detection and distribution of food-borne bacteria in ready-to-eat foods in Korea. Food Science and Biotechnology 20(2): 525.

Stoimenov PK, Klinger RL, Marchin GL and Klabunde KJ (2002). Metal oxide nanoparticles as bactericidal agents. Langmuir 18(17): 6679-6686.

Fu G, Vary PS and Lin C-T (2005). Anatase TiO2 nanocomposites for antimicrobial coatings. The journal of physical chemistry B 109(18): 8889-8898.

Bajpai SK, Chand N and Chaurasia V (2012). Nano zinc oxide-loaded calcium alginate films with potential antibacterial properties. Food and Bioprocess Technology 5(5): 1871-1881.

Haneefa M, Jayandran M and Balasubramanian M (2017). Evaluation of antimicrobial activity of green-synthesized manganese oxide nanoparticles and comparative studies with curcuminaniline functionalized nanoform. Asian J. Pharm. Clin. Res 10: 347-352.

Usha R, Prabu E, Palaniswamy M, Venil CK and Rajendran R (2010). Synthesis of metal oxide nano particles by Streptomyces sp. for development of antimicrobial textiles. Global J Biotechnol Biochem 5(3): 153-160.

Gurgur, E., Oluyamo, S., Adetuyi, A., Omotunde, O., & Okoronkwo, A. (2020). Green synthesis of zinc oxide nanoparticles and zinc oxide–silver, zinc oxide–copper nanocomposites using Bridelia ferruginea as biotemplate. SN Applied Sciences, 2(5), 1-12.

Veerakumar, K., Govindarajan, M., & Hoti, S. (2014). Evaluation of plant-mediated synthesized silver nanoparticles against vector mosquitoes. Parasitology research, 113(12), 4567-4577.

Sindhura, K. S., Prasad, T., Selvam, P. P., & Hussain, O. (2014). Synthesis, characterization and evaluation of effect of phytogenic zinc nanoparticles on soil exo-enzymes. Applied Nanoscience, 4(7), 819-827.

Paul D, Mangla S, Neogi S. Antibacterial study of CuO-NiO-ZnO trimetallic oxide nanoparticle. Materials Letters. 2020 Jul 15;271:127740.

Bala, N., Saha, S., Chakraborty, M., Maiti, M., Das, S., Basu, R., & Nandy, P. (2015). Green synthesis of zinc oxide nanoparticles using Hibiscus subdariffa leaf extract: effect of temperature on synthesis, anti-bacterial activity, and anti-diabetic activity. RSC Advances, 5(7), 4993-5003.

Kokila T, Ramesh PS, Geetha D. Biosynthesis of silver nanoparticles from Cavendish banana peel extract and its antibacterial and free radical scavenging assay: a novel biological approach. Applied Nanoscience. 2015 Nov;5(8):911-20.

How to Cite
Gillani SS, Khan SA, Nazir R, Qurashi AW. Green Synthesis of Mixed Metal Oxide (MnO, CuO, ZnO) Nanoparticles (NPs) using Rose Petal Extract: An investigation of their Antimicrobial and Antifungal Activities. Sci Inquiry Rev. [Internet]. 2021Dec.16 [cited 2024Apr.15];5(4). Available from:
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