Antioxidant and Anticancer Activity of Tannins Isolated from Callus Cultures of Achyranthes aspera L.

  • Madieha Ambreen School of Botany, Minhaj University, Lahore, Pakistan
  • Safdar Ali Mirza Botany Department, Government College University, Lahore, Pakistan
  • Zahida Bano 3Heinrich Heine University, Düsseldorf, Germany
  • Wasiq Ikram 1. School of Botany, Minhaj University, Lahore, Pakistan 2.Chair of Global Critical Zone Science, Mohammed VI Polytechnic University, Ben Guerir, Morocco
  • Muhammad Nazim Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, China
  • Syed Muhammad Waqas Gillani Pakistan Tobacco Board, Ministry of National Food Security and Research, Pakistan
  • Muhammad Talha Hassan Superior University, Lahore
  • Sajida Begum Botany Department, Government College University, Lahore Pakistan
Keywords: anticancer activity, antioxidant activity, biomass production, callus culture, cell viability, jurket cell line, medicinal plant, oxidative stress, scavenging activity, tannins content


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In the current study, the impact of oxidative stress induced by free radicals generated during metabolic processes was investigated. It was found to be linked to a variety of diseases due to their detrimental effects on nucleic acids and proteins. The primary focus remained on investigating the free radical-scavenging properties and potential anticancer activities of tannins found in callus cultures of A. aspera. It was observed that the induction of callus formation was notably successful when using leaf and root explants, resulting in a callus index as high as 160, as compared to stem explants in the presence of auxins. The extraction yield of callus tannins was the most abundant in chloroform extracts, although the overall antioxidant activity was comparable among chloroform, methanol, and petroleum ether callus extracts. Among these extracts, the chloroform extract from stem callus cultures grown on the MSDN medium showed the highest total antioxidant activity. Notably, tannins extracted from A. aspera leaf callus extracts displayed significant anticancer effects against the Jurket cell line. These effects were evaluated through measures such as cell viability and colony formation. The anticancer activity was notably higher in callus culture extracts as compared to the control. In summary, the results showed that in vitro biomass production can be a valuable approach for augmenting the production of bioactive compounds, such as tannins, from selected medicinal plants including A. aspera. Tannins extracted from A. aspera hold promise for their potential role in the development of new medicines.


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Author Biography

Madieha Ambreen, School of Botany, Minhaj University, Lahore, Pakistan

Assistant Professor, 

Minhaj University Lahore


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How to Cite
Ambreen M, Mirza SA, Bano Z, Ikram W, Nazim M, Gillani SMW, Hassan MT, Begum S. Antioxidant and Anticancer Activity of Tannins Isolated from Callus Cultures of Achyranthes aspera L. Sci Inquiry Rev. [Internet]. 2024Jul.1 [cited 2024Jul.16];8(2):1-21. Available from:
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