Antimicrobial Susceptibility of Phytoextraction in Combination with Antibiotics Against Bacteria Isolated from Diabetic Foot Infection
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The worldwide growing antibiotic resistance against microbes has shifted the trend towards medicinal plants. Herbs are gaining courtesy for their effectiveness, safety, cheapness, and therapeutic potential. The current study focuses on the antibacterial potential of Mentha piperita (mint), Syzygium cumini (jamun), Citrus limon (lemon), and Punica granatum (pomegranate) extracts against isolated bacterial strains from diabetic foot infections. Diabetic foot samples (n=80) were collected for isolation of bacteria. Samples were run on respective media for isolation of different bacteria, which were further identified through microscopy and biochemical testing. Antibacterial activity was determined by disc and well diffusion methods. Several bacteria like S. aureus (42), P. aeruginosa (41), E. coli (28) K. pneumonia (22), S. epidermidis (15), Bacillus (7), and Proteous mirabilis (7) were isolated. Antibacterial analysis revealed moderate sensitivity of the four selected extracts against isolates, which was higher compared to most synthetic antibiotics. The lemon extract showed moderate antibacterial activity against S. epidermidis (8mm), P. aeruginosa (10mm) Bacillus (10mm), and P. mirabilis (11mm). Similarly, pomegranate also showed moderate sensitivity against all isolated bacteria. Jamun leaf extract showed antibacterial activity against S. epidermidis (12mm), K. pneumonia (10mm), E. coli (12mm), S. aureus, (11mm) and P. mirabilis (10mm). Hence plants could be used as an alternative to synthetic antibiotics with modifications to achieve better results.
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Shaheen MM, Al Dahab S, Abu Fada M, Idieis R. Isolation and characterization of bacteria from diabetic foot ulcer: amputation, antibiotic resistance and mortality rate. Int J Diabet Develop Count. 2021;42:1–9. https://doi.org/10.1007/s13410-021-00997-7
Boulton AJ, Armstrong DG, Kirsner RS, et al. Diagnosis and management of diabetic foot complications. ADA Clinic Compend. 2018;2018(2):1–20. https://doi.org/10.2337/db20182-1
Care D. Economic costs of diabetes in the US in 2017. Diabetes Care. 2018;41(5):917–928. https://doi.org/10.2337/dci18-0007
Mureithi M, Ngugi N, Otieno F. Diabetic foot ulcers in a kenyan referral and teaching hospital: risk factors, patient characteristics and clinical outcomes. Series Endocrinol Diabet Metabol. 2019;1(2):41–51.
Bahadar H, Mostafalou S, Abdollahi M. Growing burden of diabetes in Pakistan and the possible role of arsenic and pesticides. J Diabet Metabol Disord. 2014;13:1–8. https://doi.org/10.1186/s40200-014-0117-y
Yan X, Song J-f, Zhang L, Li X. Analysis of risk factors for multidrug-resistant organisms in diabetic foot infection. BMC Endocr Disord. 2022;22:e46. https://doi.org/10.1186/s12902-022-00957-0
Permana R, Tosida ET, Suriansyah MI. Development of augmented reality portal for medicininal plants introduction. Int J Global Oper Res. 2022;3(2):52–63.
Ramírez-Gómez XS, Jiménez-García SN, Campos VB, Campos MLG. Plant metabolites in plant defense against pathogens. In: Topolovec-Pintaric S, ed. Plant Diseases-Current Threats and Management Trends. BoD - Books on Demand; 2019:49–68.
Oguntibeju OO. Type 2 diabetes mellitus, oxidative stress and inflammation: examining the links. Int J Phys Pathophysiol Pharmacol. 2019;11(3):45–63.
Karmaker M, Sanyal SK, Sultana M, Hossain M. Association of bacteria in diabetic and non-diabetic foot infection–An investigation in patients from Bangladesh. J Infect Public Health. 2016;9(3):267–277. https://doi.org/10.1016/j.jiph.2015.10.011
Aorkwagh M, Wanen I, Alye C, Gberindyer A, Asue U. Isolation and identification of bacteria prevalent among Nigerian currency notes in selected areas in and around Akperan Orshi College Of Agriculture, Yandev. J Sci Indust Stud. 2015;13(3):74–81.
Gonelimali FD, Lin J, Miao W, et al. Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Front Microbiol. 2018;9:e1639. https://doi.org/10.3389/fmicb.2018.01639
Manikandan C, Amsath A. Antibiotic susceptibility of bacterial strains isolated from patients with respiratory tract infections. Int J Pure Appl Zool. 2013;1(1):61–69.
Fralick M, Jenkins AJ, Khunti K, Mbanya JC, Mohan V, Schmidt MI. Global accessibility of therapeutics for diabetes mellitus. Nat Rev Endocrinol. 2022;18(4):199–204. https://doi.org/10.1038/s41574-021-00621-y
Sekhar SM, Vyas N, Unnikrishnan M, Rodrigues G, Mukhopadhyay C. Antimicrobial susceptibility pattern in diabetic foot ulcer: a pilot study. Ann Med Health Sci Res. 2014;4(5):742–745.
Karmakar M, Katsnelson MA, Dubyak GR, Pearlman E. Neutrophil P2X7 receptors mediate NLRP3 inflammasome-dependent IL-1β secretion in response to ATP. Nature Commun. 2016;7(1):e10555. https://doi.org/10.1038/ncomms10555
Appapalam ST, Muniyan A, Mohan KV, Panchamoorthy R. A study on isolation, characterization, and exploration of multiantibiotic-resistant bacteria in the wound site of diabetic foot ulcer patients. Int J Lower Extrem Wounds. 2021;20(1):6–14. https://doi.org/10.1177/15347346198844
Naeem F, Anjum FR, Arshad MA, et al. Isolation and antibiotic sensitivity pattern of drug resistant bacteria in ulcerative foot of type 2 diabetic patients. Pak J Pharm Sci. 2019;32(4):1843–1848.
Saeed S, Tariq P. Antimicrobial activities of Emblica officinalis and Coriandrum sativum against gram positive bacteria and Candida albicans. Pak J Bot. 2007;39(3):913–917.
Verma P. A study on isolation of different type of bacteria from pus. Int J Pharm Life Sci. 2012;3(11):2107–2110.
Rajalakshmi V, Amsaveni V. Antibiotic susceptibility of bacterial pathogens isolated from diabetic patients. Int J Microbiol Res. 2012;3(1):30–32.
Khadir MK, Hasan AY, Salman RM. Inhibitory effect of myrtus communis L. and syzygium aromaticum L. Extracts on the growth of staphylococcus aureus isolated from foot ulcers of diabetic patients. Diyal J Pure Sci. 2018;14(3):108–121.
Hena J, Growther L. Studies on bacterial infections of diabetic foot ulcer. Afr J Clinic Exper Microbiol. 2010;11(3):146–149. https://doi.org/10.4314/ajcem.v11i3.57768
Al-Sum BA, Al-Arfaj AA. Antimicrobial activity of the aqueous extract of mint plant. Sci J Clin Med. 2013;2(3):110–113. https://doi.org/10.11648/j.sjcm.20130203.19
Gopal PV. Evaluation of anti-microbial activity of citrus aurantium against some gram positive and negative bacterial strains. Pharmacia. 2012;1(3):107–109.
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