In vitro, In vivo, and In silico Investigation of Methanolic Extract of Withania Coagulans (Stocks) against Diarrhea and Pyretic Albino Rats
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The current research aimed to explore the potential anti-diarrheal and antipyretic properties of the Withania coagulans plant. To assess the quality and purity of the plant material, sensory analysis and physicochemical tests, including measurements of ash values, moisture content, and extractive values, were carried out. The methanolic extract of the plant was investigated for its effectiveness in reductreating diarrhea, influencing gastrointestinal motility, and countering entero-pooling induced by castor oil, and reducing fever. These investigations were conducted using doses of 250 and 500 mg/kg for anti-diarrheal and gastrointestinal motility tests, and 200 and 400 mg/kg for the antipyretic tests. The experiments were conducted on Wistar albino rats, with standard substances, such as atropine (3 mg/kg) and paracetamol (150 mg/kg) being employed as benchmarks for anti-diarrheal and anti-pyretic comparisons, respectively. Gas Chromatography-Mass Spectrometry (GC-MS) analysis uncovered the presence of diverse phytochemical compounds within the plant. Through in silico analysis of these isolated compounds using GCMS, it was determined that they exhibit binding affinity with COX and M3 receptors. The results indicated that the plant demonstrated significant anti-fever effects at 200 and 400 mg/kg doses. Moreover, its impact on treating diarrhea was more pronounced at doses of 250 and 500 mg/kg.
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